Supravital blood studies, using acridine orange fluorescence.

Supravital Blood Studies, Using Acridine Orange Fluorescence

LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...

Euchrysine, a supravital fluorescent lysosomal stain: technic and application for hematologic investigation.

Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations.

An insight to optical studies of acridine orange cationic dye within nanometer-sized microemulsions at fixed water content

Cationic dye Acridine orange (AO) has wide applications especially in biologicalfields such as analysis of lysosomal and mitochondria content by flow cytometryand so on. In the current work, spectroscopy of acridine orange (AO) dye at bothlow concentrations (mdye/mwater=6.25×10-5, 3.12×10-5) and high concentrations(mdye/mwater=0.002, 0.001) was studied in confined water ...

Urinalysis: current status and prospects for the future.

More than 300 million routine clinical analyses are performed annually in the United States. Methods for routine clinical urine examination, including detection of bacteriuria, are briefly reviewed. Prospects of some newer, better techniques to carry out such analyses are introduced. A preliminary report is presented on the use of supravital microscopic fluorescence technique (SMFT), employing ...